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AAK1 antibody (AA 186-216)

Details for Product anti-AAK1 Antibody No. ABIN652404, Supplier: Log in to see
Antigen
  • 5530400K14Rik
  • 9630042K20
  • AU067724
  • AU067726
  • BC028270
  • C79663
  • D6Ertd245e
  • R75501
  • mKIAA1048
  • RGD1563580
Epitope
AA 186-216, N-Term
22
19
15
14
10
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
88
62
41
3
1
1
1
1
1
Host
Rabbit
88
13
6
Clonality (Clone)
Polyclonal ()
Conjugate
This AAK1 antibody is un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
94
52
19
13
11
7
5
5
2
1
Supplier
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Immunogen This AAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-216 AA from the N-terminal region of human AAK1.
Clone RB16487
Isotype Ig
Specificity This AAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194~223 amino acids from the n-terminal region of human AAK1.
Predicted Reactivity Cow (Bovine),Pig (Porcine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. AAK1 is a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin.
Synonyms: AP2-associated protein kinase 1, Adaptor-associated kinase 1, AAK1, KIAA1048
Molecular Weight 93578 DA
Gene ID 22848
UniProt Q2M2I8
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-AAK1 antibody (AA 186-216) (ABIN652404) Western blot analysis of AAK1 Antibody (N-term) (ABIN652404) in Ramos cell line lysat...
Background publications Henderson, Conner: "A novel AAK1 splice variant functions at multiple steps of the endocytic pathway." in: Molecular biology of the cell, Vol. 18, Issue 7, pp. 2698-706, 2007 (PubMed).

Takahashi, Furuchi, Naganuma: "Endocytic Ark/Prk kinases play a critical role in adriamycin resistance in both yeast and mammalian cells." in: Cancer research, Vol. 66, Issue 24, pp. 11932-7, 2006 (PubMed).