NFKBIA ( S32/36 ) antibody

Antigen: NFKBIA ( S32/36 )

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652417

Additional Information

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Gene ID: 4792

Swiss-Prot: P25963

Immunogen: This NFKBIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19~49 amino acids from human NFKBIA.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI

Molecular Weight: 35478 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Signaling, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of NFKBIA-S32/36 (ABIN652417) in Hela cell line lysates (35ug/lane). NFKBIA (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with NFKBIA Antibody (S32/36 ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of hela cells using NFKBIA Antibody ( S32/36 )(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.