Actinin, alpha 1 (ACTN1) (N-Term), (AA 1-30) antibody

Details for Product No. ABIN652451
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Antigen
Epitope
N-Term, AA 1-30
(14), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(41), (14), (14), (5), (3), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(30), (18)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(46), (19), (13), (9), (7), (7), (7), (5), (5), (1), (1)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN652451
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Immunogen This ACTN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human ACTN1.
Clone RB21901
Isotype Ig
Specificity This ACTN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human ACTN1.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Monkey,Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ACTN1
Background ACTN1 is a F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Synonyms: Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, Non-muscle alpha-actinin-1, F-actin cross-linking protein, ACTN1
Molecular Weight 103058 DA
Gene ID 87
UniProt P12814
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.46 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Actinin, alpha 1 (ACTN1) (N-Term), (AA 1-30) antibody Western blot analysis of ACTN1 Antibody (N-term) (ABIN652451) in MDA-MB231 cell line lysates (35 µg/lane). ACTN1 (arrow) was detected using the purified polyclonal antibody.
anti-Actinin, alpha 1 (ACTN1) (N-Term), (AA 1-30) antibody (2) Formalin-fixed and paraffin-embedded human breast carcinoma with ACTN1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Actinin, alpha 1 (ACTN1) (N-Term), (AA 1-30) antibody (3) Flow cytometric analysis of MDA-231 cells using ACTN1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Zhang, Lin, Neel et al.: "Phosphorylated alpha-actinin and protein-tyrosine phosphatase 1B coregulate the disassembly of the focal adhesion kinase x Src complex and promote cell migration." in: The Journal of biological chemistry, Vol. 281, Issue 3, pp. 1746-54, 2006 (PubMed).

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