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Interleukin 8 (IL8) (C-Term), (AA 72-99) antibody

Details for Product No. ABIN652552
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Antigen
Synonyms IL8, K60, interleukin-8, il8, si:dkey-151b16.2, cxcli2, Il8, Scyb15, lungkine, weche, CXCL8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, IL-8, AMCF-I, EMF-1
Epitope
C-Term, AA 72-99
(21), (19), (7), (6), (5), (4), (4), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(277), (20), (20), (14), (14), (13), (11), (6), (6), (5), (5), (5), (5), (3), (2), (1)
Host
Rabbit
(173), (151), (6), (5), (4), (4), (3)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(29), (19), (6), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
(235), (231), (66), (43), (39), (29), (25), (22), (19), (17), (14), (12), (7), (5), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Pubmed 2 references available
Quantity 400 µL
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Catalog No. ABIN652552
291.50 $
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Immunogen This IL8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-99 AA from the C-terminal region of human IL8.
Clone RB20676
Isotype Ig
Specificity This IL8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70~99 amino acids from the C-terminal region of human IL8.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name IL8
Background IL8 is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor.
Synonyms: Interleukin-8, IL-8, C-X-C motif chemokine 8, Monocyte-derived neutrophil chemotactic factor, MDNCF, T-cell chemotactic factor, Neutrophil-activating protein 1, NAP-1, Protein 3-10C, Granulocyte chemotactic protein 1, GCP-1, Monocyte-derived neutrophil-activating peptide, MONAP, Emoctakin, MDNCF-a, IL8/NAP1 form I, GCP/IL-8 protein IV, Interleukin-8, IL-8(1-77), MDNCF-b, IL8/NAP1 form II, GCP/IL-8 protein II, (Ala-IL-8)77, IL-8(5-77), IL-8(6-77), Lymphocyte-derived neutrophil-activating factor, LYNAP, Neutrophil-activating factor, NAF, MDNCF-c, IL8/NAP1 form III, GCP/IL-8 protein I, (Ser-IL-8)72, IL-8(7-77), IL8/NAP1 form IV, GCP/IL-8 protein V, IL-8(8-77), IL8/NAP1 form V, GCP/IL-8 protein VI, IL-8(9-77), IL8/NAP1 form VI, GCP/IL-8 protein III , CXCL8, IL8
Pathways: Hepatitis C, Cytokine-cytokine receptor interaction, Pathways in cancer, Chemokine signaling pathway, Toll-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Malaria, Bladder cancer, Rheumatoid arthritis, Epithelial cell signaling in Helicobacter pylori infection, Chagas disease (American trypanosomiasis), Amoebiasis, Shigellosis
Molecular Weight 11098 Da
Gene ID 3576
UniProt P10145
Application Notes WB = 1:1000, IHC = 1:10-50, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Interleukin 8 (IL8) (C-Term), (AA 72-99) antibody Western blot analysis of IL8 Antibody (C-term) (ABIN652552) in MDA-MB468 cell line lysates (35 µg/lane). IL8 (arrow) was detected using the purified polyclonal antibody.
anti-Interleukin 8 (IL8) (C-Term), (AA 72-99) antibody (2) Formalin-fixed and paraffin-embedded human lung carcinoma reacted with IL8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Interleukin 8 (IL8) (C-Term), (AA 72-99) antibody (3) IL8 Antibody (C-term) (ABIN652552) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Baggiolini, Clark-Lewis: "Interleukin-8, a chemotactic and inflammatory cytokine." in: FEBS letters, Vol. 307, Issue 1, pp. 97-101, 1992 (PubMed).

Holmes, Lee, Kuang et al.: "Structure and functional expression of a human interleukin-8 receptor." in: Science (New York, N.Y.), Vol. 253, Issue 5025, pp. 1278-80, 1991 (PubMed).

Validation Images
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