Microtubule Associated Protein 6 (MAP6) (Center) antibody

Antigen: Microtubule Associated Protein 6 (MAP6)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652614

Additional Information

Alternative name: MAP6

Gene ID: 4135

UniProt: Q96JE9

Immunogen: This MAP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222~251 amino acids from the Center region of human MAP6.

Isotype: Ig

Description: Other names: Microtubule-associated protein 6, MAP-6, Stable tubule-only polypeptide, STOP, KIAA1878, MAP6

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MAP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222~251 amino acids from the Center region of human MAP6.

Molecular Weight: 47,376 DA

Comments:

Background: MAP6 is a microtubule-associated protein. This protein is a calmodulin-binding and calmodulin-regulated protein that is involved in microtubule stabilization.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology

Restrictions: For Research Use only

Images

Western blot analysis of MAP6 Antibody (Center) (ABIN652614) in HL-60 cell line lysates (35ug/lane). MAP6 (arrow) was detected using the purified Pab.

Western blot analysis of MAP6 Antibody (Center) (ABIN652614) in NIH-3T3 cell line lysates (35ug/lane). MAP6 (arrow) was detected using the purified Pab.

MAP6 Antibody (Center) (ABIN652614) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Andrieux, Salin, Vernet et al.: "The suppression of brain cold-stable microtubules in mice induces synaptic defects associated with neuroleptic-sensitive behavioral disorders." in: Genes & development, Vol. 16, Issue 18, pp. 2350-64, 2002 (PubMed).

Bosc, Andrieux, Job: "STOP proteins." in: Biochemistry, Vol. 42, Issue 42, pp. 12125-32, 2003 (PubMed).