Engulfment and Cell Motility 1 (ELMO1) (Center) antibody

Details for Product No. ABIN652707
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Antigen
Epitope
Center
(9), (9), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(41), (19), (15), (15), (14), (12), (3), (1), (1), (1), (1)
Host
Rabbit
(34), (8), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS)
(34), (16), (10), (5), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 400 μL
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Catalog No. ABIN652707
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Immunogen This ELMO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 337~366 amino acids from the Center region of human ELMO1.
Specificity This ELMO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 337~366 amino acids from the Center region of human ELMO1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ELMO1
Background ELMO1 interacts with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. Similarity to a C. elegans protein suggests that this protein may function in apoptosis and in cell migration.
Synonyms: Protein ced-12 homolog, Engulfment and cell motility protein 1, ELMO1, KIAA0281
Molecular Weight 28,742 DA
Gene ID 9844
UniProt Q92556
Research Area Cell Structure
Application Notes The suggested dilution is: Western blotting= 1:100-500 ,Flow cytometry = 1:10-50
Comment

Background: ELMO1 interacts with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. Similarity to a C. elegans protein suggests that this protein may function in apoptosis and in cell migration.

Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Engulfment and Cell Motility 1 (ELMO1) (Center) antibody Western blot analysis of ELMO1 Antibody (Center) (ABIN652707) in Ramos cell line lysates (35 µg/lane). ELMO1 (arrow) was detected using the purified polyclonal antibody.
anti-Engulfment and Cell Motility 1 (ELMO1) (Center) antibody (2) ELMO1 Antibody (Center) (ABIN652707) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Choudhary, Kumar, Gnad et al.: "Lysine acetylation targets protein complexes and co-regulates major cellular functions." in: Science (New York, N.Y.), Vol. 325, Issue 5942, pp. 834-40, 2009 (PubMed).

Validation Images
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