Engulfment and Cell Motility 1 (ELMO1) (Center) antibody

Antigen: Engulfment and Cell Motility 1 (ELMO1)

Synonyms: CED12, CED-12, ELMO-1, KIAA0281, MGC126406, ELMO1, MGC68475, zgc:85612, im:7151147, wu:fq24e08, MGC139838, DKFZp459C0528

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652707

Additional Information

Alternative name: ELMO1

Gene ID: 9844

UniProt: Q92556

Immunogen: This ELMO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 337~366 amino acids from the Center region of human ELMO1.

Isotype: Ig

Description: Other names: Protein ced-12 homolog, Engulfment and cell motility protein 1, ELMO1, KIAA0281

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ELMO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 337~366 amino acids from the Center region of human ELMO1.

Molecular Weight: 28,742 DA

Comments:

Background: ELMO1 interacts with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. Similarity to a C. elegans protein suggests that this protein may function in apoptosis and in cell migration.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ELMO1 Antibody (Center) (ABIN652707) in Ramos cell line lysates (35ug/lane). ELMO1 (arrow) was detected using the purified Pab.

ELMO1 Antibody (Center) (ABIN652707) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Choudhary, Kumar, Gnad et al.: "Lysine acetylation targets protein complexes and co-regulates major cellular functions." in: Science (New York, N.Y.), Vol. 325, Issue 5942, pp. 834-40, 2009 (PubMed).