Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A) (Center) antibody

Antigen: Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A)

Synonyms: ENaCa, SCNEA, SCNN1, FLJ21883, ENaCalpha, ENaC, Scnn1, mENaC, SCNN1A

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652752

Additional Information

Alternative name: SCNN1A

Gene ID: 6337

UniProt: P37088

Immunogen: This SCNN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 371~400 amino acids from the Center region of human SCNN1A.

Isotype: Ig

Description: Other names: Alpha-NaCH, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA, Epithelial Na(+) channel subunit alpha, Alpha-ENaC, ENaCA, Amiloride-sensitive sodium channel subunit alpha, SCNN1A, SCNN1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SCNN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 371~400 amino acids from the Center region of human SCNN1A.

Molecular Weight: 28,328 DA

Comments:

Background: Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been sociated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of SCNN1A Antibody (Center) (ABIN652752) in WiDr cell line lysates (35ug/lane). SCNN1A (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with SCNN1A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of SCNN1A Antibody (Center) (ABIN652752) with Hela cell followed by Alexa Fluor® 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Publications

Product: Azad, Rauh, Vermeulen et al.: "Mutations in the amiloride-sensitive epithelial sodium channel in patients with cystic fibrosis-like disease." in: Human mutation, Vol. 30, Issue 7, pp. 1093-103, 2009 (PubMed).