EF-Hand Domain Family, Member A1 (EFHA1) (Center) antibody

Antigen: EF-Hand Domain Family, Member A1 (EFHA1)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652818

Additional Information

Alternative name: EFHA1

Gene ID: 221154

UniProt: Q8IYU8

Immunogen: This EFHA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233~262 amino acids from the Center region of human EFHA1.

Isotype: Ig

Description: Other names: EF-hand domain-containing family member A1, EFHA1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EFHA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233~262 amino acids from the Center region of human EFHA1.

Molecular Weight: 49666 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of EFHA1 Antibody (Center) (ABIN652818) in MDA-MB231 cell line lysates (35ug/lane). EFHA1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with EFHA1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

EFHA1 Antibody (Center) (ABIN652818) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Dunham, Matthews, Burton et al.: "The DNA sequence and analysis of human chromosome 13." in: Nature, Vol. 428, Issue 6982, pp. 522-8, 2004 (PubMed).