Ankyrin Repeat Domain 24 (ANKRD24) (C-Term) antibody

Antigen: Ankyrin Repeat Domain 24 (ANKRD24)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652879

Additional Information

Alternative name: ANKRD24

Gene ID: 170961

UniProt: Q8TF21

Immunogen: This ANKRD24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1042~1072 amino acids from the C-terminal region of human ANKRD24.

Isotype: Ig

Description: Other names: Ankyrin repeat domain-containing protein 24, ANKRD24, KIAA1981

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ANKRD24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1042~1072 amino acids from the C-terminal region of human ANKRD24.

Molecular Weight: 124187 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ANKRD24 Antibody (C-term) (ABIN652879) in mouse cerebellum tissue lysates (35ug/lane). ANKRD24 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with ANKRD24 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

ANKRD24 Antibody (C-term) (ABIN652879) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Brandenberger, Wei, Zhang et al.: "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation." in: Nature biotechnology, Vol. 22, Issue 6, pp. 707-16, 2004 (PubMed).