TBP-Associated Factor 172 (BTAF1) (C-Term) antibody

Antigen: TBP-Associated Factor 172 (BTAF1)

Synonyms: MOT1, TAF172, KIAA0940, TAFII170, MGC138406, TAF(II)170

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652913

Additional Information

Alternative name: BTAF1

Gene ID: 9044

UniProt: O14981, NP_003963

Immunogen: This BTAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1823-1852 amino acids from the C-terminal region of human BTAF1.

Isotype: Ig

Description: Other names: TATA-binding protein-associated factor 172,BTAF1,TAF172

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BTAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1823-1852 amino acids from the C-terminal region of human BTAF1.

Molecular Weight: 206887 DA

Comments:

Background: Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP, MIM 600075) and TBP-associated factors, or TAFs (e.g., TAF2B, MIM 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Restrictions: For Research Use only

Images

BTAF1 Antibody (C-term) (ABIN652913) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the BTAF1 antibody detected the BTAF1 protein (arrow).

BTAF1 Antibody (C-term) (ABIN652913) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BTAF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

BTAF1 Antibody (C-term) (ABIN652913) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Grupe, Li, Rowland et al.: "A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease." in: American journal of human genetics, Vol. 78, Issue 1, pp. 78-88, 2005 (PubMed).

Grob, Cruse, Inouye et al.: "Cryo-electron microscopy studies of human TFIID: conformational breathing in the integration of gene regulatory cues." in: Structure (London, England : 1993), Vol. 14, Issue 3, pp. 511-20, 2006 (PubMed).

Koutroubas, Merika, Thanos: "Bypassing the requirements for epigenetic modifications in gene transcription by increasing enhancer strength." in: Molecular and cellular biology, Vol. 28, Issue 3, pp. 926-38, 2008 (PubMed).