Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3) (Center) antibody

Antigen: Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3)

Synonyms: SLC12A3, slc12a3, DKFZp469N2315, TSC, NCCT, FLJ96318

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652920

Additional Information

Alternative name: SLC12A3

Gene ID: 6559

UniProt: P55017

Immunogen: This SLC12A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 791~820 amino acids from the Center region of human SLC12A3.

Isotype: Ig

Description: Other names: Na-Cl symporter, Thiazide-sensitive sodium-chloride cotransporter, Solute carrier family 12 member 3, SLC12A3, TSC

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SLC12A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 791~820 amino acids from the Center region of human SLC12A3.

Molecular Weight: 113125 DA

Comments:

Background: SLC12A3 is a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Restrictions: For Research Use only

Images

Western blot analysis of SLC12A3 Antibody (Center) (ABIN652920) in HL-60 cell line lysates (35ug/lane). SLC12A3 (arrow) was detected using the purified Pab.

SLC12A3 Antibody (Center) (ABIN652920) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Simon, Nelson-Williams, Bia et al.: "Gitelman's variant of Bartter's syndrome, inherited hypokalaemic alkalosis, is caused by mutations in the thiazide-sensitive Na-Cl cotransporter." in: Nature genetics, Vol. 12, Issue 1, pp. 24-30, 1996 (PubMed).

Mastroianni, De Fusco, Zollo et al.: "Molecular cloning, expression pattern, and chromosomal localization of the human Na-Cl thiazide-sensitive cotransporter (SLC12A3)." in: Genomics, Vol. 35, Issue 3, pp. 486-93, 1996 (PubMed).