BARX Homeobox 2 (BARX2) (Center) antibody

Antigen: BARX Homeobox 2 (BARX2)

Synonyms: MGC133368, MGC133369, Barx2b, 2310006E12Rik, Barh, MBH1, MGC90874, E130309B19Rik, Mbh2, Barhl2, BARX2B, xbh, XBH1, barh1, xbarhl2, BARX2, BARHL2, BarH3, wu:fc90f05, zgc:112193, B-H2, MGC113948, wu:fq39g04, zgc:113948

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652927

Additional Information

Alternative name: BARX2

Gene ID: 8538

UniProt: Q9UMQ3

Immunogen: This BARX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116~145 amino acids from the Center region of human BARX2.

Isotype: Ig

Description: Other names: Homeobox protein BarH-like 2, BARX2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BARX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116~145 amino acids from the Center region of human BARX2.

Molecular Weight: 31188 DA

Comments:

Background: BARX2 is a transcription factor. It binds optimally to the DNA consensus sequence 5'-YYTAATGRTTTTY-3'. May control the expression of neural adhesion molecules such as L1 or Ng-CAM during embryonic development of both the central and peripherical nervous system. May be involved in controlling adhesive processes in keratinizing epithelia (By similarity).

Synonyms: MGC133368, MGC133369, Barx2b, 2310006E12Rik, Barh, MBH1, MGC90874, E130309B19Rik, Mbh2, Barhl2, BARX2B, xbh, XBH1, barh1, xbarhl2, BARX2, BARHL2, BarH3, wu:fc90f05, zgc:112193, B-H2, MGC113948, wu:fq39g04, zgc:113948

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Transcription Factors, Signaling, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of BARX2 Antibody (Center) (ABIN652927) in mouse liver tissue lysates (35ug/lane). BARX2 (arrow) was detected using the purified Pab.

BARX2 Antibody (Center) (ABIN652927) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).