ADHFE1 (Center) antibody

Antigen: ADHFE1 (Center)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653005

Additional Information

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Gene ID: 137872

Swiss-Prot: Q8IWW8

Immunogen: This ADHFE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283~312 amino acids from the Center region of human ADHFE1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Alcohol dehydrogenase iron-containing protein 1, ADHFe1, Fe-containing alcohol dehydrogenase, Hydroxyacid-oxoacid transhydrogenase, mitochondrial, HOT, HMFT2263, ADHFE1

Molecular Weight: 50308 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer

Restrictions: For Research Use only

Images

Western blot analysis of ADHFE1 Antibody (Center) (ABIN653005) in mouse heart tissue lysates (35ug/lane). ADHFE1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ADHFE1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.