Minichromosome Maintenance Complex Component 5 (MCM5) (C-Term) antibody

Antigen: Minichromosome Maintenance Complex Component 5 (MCM5)

Synonyms: CDC46, MGC5315, P1-CDC46, Cdc46, Mcmd5, mCD46, mCDC46, AA617332, AI324988, AL033333, mcm5-b, MGC68977, mcm5, MGC97563, 23.m06024

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN653020

Additional Information

Alternative name: MCM5

Gene ID: 4174

UniProt: NP_006730

Immunogen: This MCM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 705~734 amino acids from the C-terminal region of human MCM5.

Isotype: Ig

Description: Other names: P1-CDC46, CDC46 homolog, DNA replication licensing factor MCM5, CDC46, MCM5

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MCM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 705~734 amino acids from the C-terminal region of human MCM5.

Molecular Weight: 82286 DA

Comments:

Background: MCM5 is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Western blot analysis of MCM5 Antibody (C-term) (ABIN653020) pre-incubated without(lane 1) and with(lane 2) blocking peptide in NCI-H460 cell line lysate. MCM5 (arrow) was detected using the purified Pab (1:60 dilution).

MCM5 Antibody (C-term) (ABIN653020) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCM5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

MCM5 Antibody (C-term) (ABIN653020) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Snyder, Huang, Zhang: "The minichromosome maintenance proteins 2-7 (MCM2-7) are necessary for RNA polymerase II (Pol II)-mediated transcription." in: The Journal of biological chemistry, Vol. 284, Issue 20, pp. 13466-72, 2009 (PubMed).

Saade, Mechold, Kulyyassov et al.: "Analysis of interaction partners of H4 histone by a new proteomics approach." in: Proteomics, Vol. 9, Issue 21, pp. 4934-43, 2009 (PubMed).