Importin 5 (IPO5) (C-Term) antibody

Antigen: Importin 5 (IPO5)

Synonyms: IPO5, RANBP5

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653053

Additional Information

Alternative name: RANBP5

Gene ID: 3843

UniProt: NP_002262, O00410

Immunogen: This RANBP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 794-823 amino acids from the C-terminal region of human RANBP5.

Isotype: Ig

Description: Other names: Importin-5,IPO5,RANBP5, KPNB3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RANBP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 794-823 amino acids from the C-terminal region of human RANBP5.

Molecular Weight: 123630 DA

Comments:

Background: Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

RANBP5 Antibody (C-term) (ABIN653053) western blot analysis in A2058 cell line lysates (15ug/lane).This demonstrates the RANBP5 antibody detected the RANBP5 protein (arrow).

RANBP5 Antibody (C-term) (ABIN653053) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RANBP5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Bukrinsky, Sharova, Dempsey et al.: "Active nuclear import of human immunodeficiency virus type 1 preintegration complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 14, pp. 6580-4, 1992 (PubMed).

Bukrinsky, Haggerty, Dempsey et al.: "A nuclear localization signal within HIV-1 matrix protein that governs infection of non-dividing cells." in: Nature, Vol. 365, Issue 6447, pp. 666-9, 1993 (PubMed).