DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 55 (DDX55) (C-Term) antibody

Antigen: DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 55 (DDX55)

Synonyms: FLJ16577, KIAA1595, MGC33209, MGC143677, MGC143678, mKIAA1595, 2810021H22Rik, fc32a12, kiaa1595, chunp6861, wu:fc32a12, ddx55, MGC78784, DDX55, MGC134525

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653108

Additional Information

Alternative name: DDX55

Gene ID: 57696

UniProt: Q8NHQ9

Immunogen: This DDX55 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495~525 amino acids from the C-terminal region of human DDX55.

Isotype: Ig

Description: Other names: DEAD box protein 55, ATP-dependent RNA helicase DDX55,KIAA1595, DDX55

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DDX55 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495~525 amino acids from the C-terminal region of human DDX55.

Molecular Weight: 68489 DA

Comments:

Background: DDX55 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications

Restrictions: For Research Use only

Images

Western blot analysis of DDX55 Antibody (C-term) (ABIN653108) in Y79 cell line lysates (35ug/lane). DDX55 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX55 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

DDX55 Antibody (C-term) (ABIN653108) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Choudhary, Kumar, Gnad et al.: "Lysine acetylation targets protein complexes and co-regulates major cellular functions." in: Science (New York, N.Y.), Vol. 325, Issue 5942, pp. 834-40, 2009 (PubMed).