Anaphase Promoting Complex Subunit 7 (ANAPC7) (Center) antibody

Antigen: Anaphase Promoting Complex Subunit 7 (ANAPC7)

Synonyms: APC7, MGC81591

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653166

Additional Information

Alternative name: ANAPC7

Gene ID: 51434

UniProt: Q9UJX3

Immunogen: This ANAPC7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272~301 amino acids from the Center region of human ANAPC7.

Isotype: Ig

Description: Other names: Cyclosome subunit 7, Anaphase-promoting complex subunit 7, APC7, APC7, ANAPC7

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ANAPC7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272~301 amino acids from the Center region of human ANAPC7.

Molecular Weight: 63133 DA

Comments:

Background: APC7 is one of the subunits of the Anaphase Promoting Complex. APC7 (anaphase-promoting complex subunit 7) is a member of the E3 enzyme family. This protein contains TPR repeats and has a molecular weight of approximately 63 kD. The APC7 protein is located in the nucleus during interphase and the centrosome during metaphase/anaphase. This protein probably recruits Cdh1 into the APC complex. The APC7 protein functions with other members of the APC complex as a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, and spindle elongation. The APC6 protein comprises one subunit of the anaphase promoting complex including APC1-8, and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ANAPC7 Antibody (Center) (ABIN653166) in K562 cell line lysates (35ug/lane). ANAPC7 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with ANAPC7 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

ANAPC7 Antibody (Center) (ABIN653166) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Nilsson, Yekezare, Minshull et al.: "The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction." in: Nature cell biology, Vol. 10, Issue 12, pp. 1411-20, 2008 (PubMed).