ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, gamma Polypeptide 1 (ATP5C1) (N-Term) antibody

Antigen: ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, gamma Polypeptide 1 (ATP5C1)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653186

Additional Information

Alternative name: ATP5C1

Gene ID: 509

UniProt: P36542

Immunogen: This ATP5C1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46~76 amino acids from the N-terminal region of human ATP5C1.

Isotype: Ig

Description: Other names: F-ATPase gamma subunit, ATP synthase subunit gamma, mitochondrial, ATP5CL1, ATP5C, ATP5C1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ATP5C1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46~76 amino acids from the N-terminal region of human ATP5C1.

Molecular Weight: 32996 DA

Comments:

Background: ATP5C1 encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ATP5C1 Antibody (N-term) (ABIN653186) in HL-60 cell line lysates (35ug/lane).ATP5C1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ATP5C1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

ATP5C1 Antibody (N-term) (ABIN653186) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Wang, McDonnell, Hebbring et al.: "Polymorphisms in mitochondrial genes and prostate cancer risk." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 17, Issue 12, pp. 3558-66, 2008 (PubMed).

Wheeler, Metter, Tanaka et al.: "Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging." in: PLoS genetics, Vol. 5, Issue 10, pp. e1000685, 2009 (PubMed).