Chromosome 20 Open Reading Frame 72 (C20orf72) (Center) antibody

Antigen: Chromosome 20 Open Reading Frame 72 (C20orf72)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653187

Additional Information

Alternative name: C20orf72

Gene ID: 92667

UniProt: Q9BQP7

Immunogen: This C20orf72 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142~171 amino acids from the Center region of human C20orf72.

Isotype: Ig

Description: Other names: Uncharacterized protein C20orf72, C20orf72

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This C20orf72 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142~171 amino acids from the Center region of human C20orf72.

Molecular Weight: 39421 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of C20orf72 Antibody (Center) (ABIN653187) in CEM cell line lysates (35ug/lane). C20orf72 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with C20orf72 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

C20orf72 Antibody (Center) (ABIN653187) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Deloukas, Matthews, Ashurst et al.: "The DNA sequence and comparative analysis of human chromosome 20." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).