Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2) (N-Term) antibody

Antigen: Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2)

Synonyms: p50, KBF1, p105, EBP-1, MGC54151, NFKB-p50, NF-kappaB, NFKB-p105, NF-kappa-B, DKFZp686C01211, p52, LYT10, LYT-10, NF-kB, NF-KB1, NFKB1, kbf1, ebp-1, MGC86262, nfkb-p50, nf-kappaB, nfkb-p105, nf-kappa-b, NFKB2, MGC179374

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653197

Additional Information

Alternative name: NFKB2

Gene ID: 18034

UniProt: Q9WTK5

Immunogen: This MOUSE NFKB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301~330 amino acids from the N-terminal region of MOUSE NFKB2.

Isotype: Ig

Description: Other names: Nuclear factor NF-kappa-B p52 subunit, DNA-binding factor KBF2, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, Nuclear factor NF-kappa-B p100 subunit, Nfkb2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MOUSE NFKB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301~330 amino acids from the N-terminal region of MOUSE NFKB2.

Molecular Weight: 96749 DA

Synonyms: p50, KBF1, p105, EBP-1, MGC54151, NFKB-p50, NF-kappaB, NFKB-p105, NF-kappa-B, DKFZp686C01211, p52, LYT10, LYT-10, NF-kB, NF-KB1, NFKB1, kbf1, ebp-1, MGC86262, nfkb-p50, nf-kappaB, nfkb-p105, nf-kappa-b, NFKB2, MGC179374

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of MOUSE NFKB2 Antibody (N-term) (ABIN653197) in A375 cell line lysates (35ug/lane). NFKB2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with MOUSE NFKB2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of MOUSE NFKB2 Antibody (N-term) (ABIN653197) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Publications

Product: Castro, Wright, Damdinsuren et al.: "B cell receptor-mediated sustained c-Rel activation facilitates late transitional B cell survival through control of B cell activating factor receptor and NF-kappaB2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 12, pp. 7729-37, 2009 (PubMed).

Guo, Huss, Tong et al.: "Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst." in: Developmental cell, Vol. 18, Issue 4, pp. 675-85, 2010 (PubMed).