Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B) (Center) antibody

Antigen: Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B)

Synonyms: PRT1, EIF3S9, EIF3-ETA, EIF3-P110, EIF3-P116, MGC104664, MGC131875, Eif3s9, AL033316, AL033334, AL033369, AW208965, D5Wsu45e, eif3s9, EIF3B, ATEIF3B-2, EIF3B-2, EUKARYOTIC TRANSLATION INITIATION FACTOR 3B, EUKARYOTIC TRANSLATION INITIATION FACTOR 3B-2, F18A17.30, F18A17_30, ARABIDOPSIS THALIANA TRANSLATION INITIATION FACTOR 3B1, ATEIF3B-1, ATTIF3B1, EIF3B-1, EUKARYOTIC TRANSLATION INITIATION FACTOR 3B1, T1G16.2, TIF3B1, TRANSLATION INITIATION FACTOR 3B1, MGC112720, MGC99017, eIF-3-eta, MGC159978, wu:fc17c01, GB10123, eIF3b

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653239

Additional Information

Alternative name: EIF3B

Gene ID: 8662

UniProt: P55884

Immunogen: This EIF3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 205~234 amino acids from the Center region of human EIF3B.

Isotype: Ig

Description: Other names: Prt1 homolog, hPrt1, eIF3 p110, eIF3 p116, eIF-3-eta, Eukaryotic translation initiation factor 3 subunit 9, Eukaryotic translation initiation factor 3 subunit B, eIF3b, EIF3S9, EIF3B

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EIF3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 205~234 amino acids from the Center region of human EIF3B.

Molecular Weight: 92482 DA

Comments:

Background: eIF3B binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The eIF3 complex is composed of at least 12 different subunits.

Synonyms: PRT1, EIF3S9, EIF3-ETA, EIF3-P110, EIF3-P116, MGC104664, MGC131875, Eif3s9, AL033316, AL033334, AL033369, AW208965, D5Wsu45e, eif3s9, EIF3B, ATEIF3B-2, EIF3B-2, EUKARYOTIC TRANSLATION INITIATION FACTOR 3B, EUKARYOTIC TRANSLATION INITIATION FACTOR 3B-2, F18A17.30, F18A17_30, ARABIDOPSIS THALIANA TRANSLATION INITIATION FACTOR 3B1, ATEIF3B-1, ATTIF3B1, EIF3B-1, EUKARYOTIC TRANSLATION INITIATION FACTOR 3B1, T1G16.2, TIF3B1, TRANSLATION INITIATION FACTOR 3B1, MGC112720, MGC99017, eIF-3-eta, MGC159978, wu:fc17c01, GB10123, eIF3b

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of EIF3B Antibody (Center) (ABIN653239) in Jurkat cell line lysates (35ug/lane). EIF3B (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with EIF3B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

EIF3B Antibody (Center) (ABIN653239) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Masutani, Sonenberg, Yokoyama et al.: "Reconstitution reveals the functional core of mammalian eIF3." in: The EMBO journal, Vol. 26, Issue 14, pp. 3373-83, 2007 (PubMed).