Asparagine-Linked Glycosylation 1, beta-1,4-Mannosyltransferase Homolog (S. Cerevisiae) (ALG1) (Center) antibody

Antigen: Asparagine-Linked Glycosylation 1, beta-1,4-Mannosyltransferase Homolog (S. Cerevisiae) (ALG1)

Synonyms: HMT1, HMAT1, HMT-1, MGC18946, zgc:66221, wu:fi34b12

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653272

Additional Information

Alternative name: ALG1

Gene ID: 56052

UniProt: Q9BT22

Immunogen: This ALG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243~272 amino acids from the Center region of human ALG1.

Isotype: Ig

Description: Other names: Asparagine-linked glycosylation protein 1 homolog, Mannosyltransferase-1, hMat-1, MT-1, Beta-1,4-mannosyltransferase, GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase, GDP-mannose-dolichol diphosphochitobiose mannosyltransferase, Chitobiosyldiphosphodolichol beta-mannosyltransferase, UNQ861/PRO1870, PSEC0061, HMT1, HMAT1, ALG1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ALG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243~272 amino acids from the Center region of human ALG1.

Molecular Weight: 52518 DA

Comments:

Background: The enzyme encoded by this protein catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. This protein is mutated in congenital disorder of glycosylation type Ik.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ALG1 Antibody (Center) (ABIN653272) in mouse kidney tissue lysates (35ug/lane). ALG1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with C10orf120 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Grubenmann, Frank, Hülsmeier et al.: "Deficiency of the first mannosylation step in the N-glycosylation pathway causes congenital disorder of glycosylation type Ik." in: Human molecular genetics, Vol. 13, Issue 5, pp. 535-42, 2004 (PubMed).

Schwarz, Thiel, Lübbehusen et al.: "Deficiency of GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase causes congenital disorder of glycosylation type Ik." in: American journal of human genetics, Vol. 74, Issue 3, pp. 472-81, 2004 (PubMed).

Kranz, Denecke, Lehle et al.: "Congenital disorder of glycosylation type Ik (CDG-Ik): a defect of mannosyltransferase I." in: American journal of human genetics, Vol. 74, Issue 3, pp. 545-51, 2004 (PubMed).