Galactose Mutarotase (Aldose 1-Epimerase) (GALM) (N-Term) antibody

Antigen: Galactose Mutarotase (Aldose 1-Epimerase) (GALM)

Synonyms: IBD1, BLOCK25, MGC93738, MUT, GALM, galm, MGC127639, DKFZp469F1113

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653294

Additional Information

Alternative name: GALM

Gene ID: 130589

UniProt: Q96C23

Immunogen: This GALM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33~63 amino acids from the N-terminal region of human GALM.

Isotype: Ig

Description: Other names: Galactose mutarotase, Aldose 1-epimerase, BLOCK25, GALM

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GALM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33~63 amino acids from the N-terminal region of human GALM.

Molecular Weight: 37766 DA

Comments:

Background: GALM encodes an enzyme that catalyzes the epimerization of hexose sugars such as glucose and galactose. The encoded protein is expressed in the cytoplasm and has a preference for galactose. The encoded protein may be required for normal galactose metabolism by maintaining the equilibrium of alpha and beta anomers of galactose.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of GALM Antibody (N-term) (ABIN653294) in mouse NIH-3T3 cell line lysates (35ug/lane). GALM (arrow) was detected using the purified Pab.

Publications

Product: Holden, Rayment, Thoden: "Structure and function of enzymes of the Leloir pathway for galactose metabolism." in: The Journal of biological chemistry, Vol. 278, Issue 45, pp. 43885-8, 2003 (PubMed).

Thoden, Timson, Reece et al.: "Molecular structure of human galactose mutarotase." in: The Journal of biological chemistry, Vol. 279, Issue 22, pp. 23431-7, 2004 (PubMed).

Pai, Chen, Zhang et al.: "Galactomutarotase and other galactose-related genes are rapidly induced by retinoic acid in human myeloid cells." in: Biochemistry, Vol. 46, Issue 51, pp. 15198-207, 2007 (PubMed).