Galanin Receptor 2 (GALR2) (Center) antibody

Antigen: Galanin Receptor 2 (GALR2)

Synonyms: GALNR2, MGC125983, MGC125984, mGalR, MGC151357, MGC151359, GALR2

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653296

Additional Information

Alternative name: GALR2

Gene ID: 8811

UniProt: O43603

Immunogen: This GALR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216~245 amino acids from the Center region of human GALR2.

Isotype: Ig

Description: Other names: Galanin receptor type 2, GAL2-R, GALR-2, GALNR2, GALR2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GALR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216~245 amino acids from the Center region of human GALR2.

Molecular Weight: 41700 DA

Comments:

Background: GALR2 is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamopituitary axis. It is a 30-amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The actions of galanin are mediated through interaction with specific membrane receptors that are members of the 7-transmembrane family of G protein-coupled receptors. GALR2 interacts with the N-terminal residues of the galanin peptide. The primary signaling mechanism for GALR2 is through the phospholipase C/protein kinase C pathway (via Gq), in contrast to GALR1, which communicates its intracellular signal by inhibition of adenylyl cyclase through Gi. However, it has been demonstrated that GALR2 couples efficiently to both the Gq and Gi proteins to simultaneously activate 2 independent signal transduction pathways.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Cancer

Restrictions: For Research Use only

Images

Western blot analysis of GALR2 Antibody (Center) (ABIN653296) in Hela cell line lysates (35ug/lane). GALR2 (arrow) was detected using the purified Pab.

GALR2 Antibody (Center) (ABIN653296) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Rikova, Guo, Zeng et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).