ATLA2 (Center) antibody

Antigen: ATLA2

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653314

Additional Information

Gene ID: 64225

UniProt: Q8NHH9

Immunogen: This ATLA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304~334 amino acids from the Center region of human ATLA2.

Isotype: Ig

Description: Other names: ADP-ribosylation factor-like protein 6-interacting protein 2, ARL-6-interacting protein 2, Aip-2, Atlastin-2, ARL6IP2, ATL2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ATLA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304~334 amino acids from the Center region of human ATLA2.

Molecular Weight: 66229 DA

Comments:

Background: ATLA2 is GTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. This protein play a role in endoplasmic reticulum tubular network biogenesis.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ATLA2 Antibody (Center) (ABIN653314) in HepG2 cell line lysates (35ug/lane). ATLA2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ATLA2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

ATLA2 Antibody (Center) (ABIN653314) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Rismanchi, Soderblom, Stadler et al.: "Atlastin GTPases are required for Golgi apparatus and ER morphogenesis." in: Human molecular genetics, Vol. 17, Issue 11, pp. 1591-604, 2008 (PubMed).

Hu, Shibata, Zhu et al.: "A class of dynamin-like GTPases involved in the generation of the tubular ER network." in: Cell, Vol. 138, Issue 3, pp. 549-61, 2009 (PubMed).