WD Repeat Domain 75 (WDR75) (C-Term) antibody

Antigen: WD Repeat Domain 75 (WDR75)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653316

Additional Information

Alternative name: WDR75

Gene ID: 84128

UniProt: Q8IWA0

Immunogen: This WDR75 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 775~805 amino acids from the C-terminal region of human ADAMTS15.

Isotype: Ig

Description: Other names: WD repeat-containing protein 75, WDR75

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This WDR75 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 775~805 amino acids from the C-terminal region of human ADAMTS15.

Molecular Weight: 94499 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications

Restrictions: For Research Use only

Images

Western blot analysis of WDR75 Antibody (C-term) (ABIN653316) in MDA-MB231 cell line lysates (35ug/lane). WDR75 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with WDR75 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Hillier, Graves, Fulton et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005 (PubMed).

Nousiainen, Silljé, Sauer et al.: "Phosphoproteome analysis of the human mitotic spindle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 14, pp. 5391-6, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).