Minichromosome Maintenance Complex Component 2 (MCM2) (AA 649-677), (C-Term) antibody

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Antigen
Synonyms
CG7538, DmMCM2, DmMcm2, DmeMCM2, Dmel\\CG7538, MCM2, MCM2-7, MCM2_DROME, McM2, PCR3, dMCM2, l(3)rL074, XMcm2, bm28, ccnl1, cdc19, cdcl1, mitotin, cb737, chunp6905, BM28, CCNL1, CDCL1, D3S3194, MITOTIN ... show more
CG7538, DmMCM2, DmMcm2, DmeMCM2, Dmel\\CG7538, MCM2, MCM2-7, MCM2_DROME, McM2, PCR3, dMCM2, l(3)rL074, XMcm2, bm28, ccnl1, cdc19, cdcl1, mitotin, cb737, chunp6905, BM28, CCNL1, CDCL1, D3S3194, MITOTIN, MCM7, AA959861, AW476101, Mcmd2, mKIAA0030 show less
Alternatives
Epitope
AA 649-677, C-Term
(38), (21), (16), (16), (15), (13), (9), (9), (8), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Reactivity
Human
(255), (78), (43), (3), (3), (3), (3), (2), (2), (1)
Host
Rabbit
(201), (73), (5), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(7), (7), (7), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(225), (99), (78), (73), (52), (29), (24), (19), (11), (10), (7), (6), (5), (3), (3), (2), (1), (1)
Pubmed 6 references available
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Quantity 400 μL
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Immunogen This MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 649-677 AA from the C-terminal region of human MCM2.
Clone RB25168
Isotype Ig
Specificity This MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656~685 amino acids from the C-terminal region of human MCM2.
Predicted Reactivity Mouse (Murine),Xenopus laevis
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name MCM2 (MCM2 Antibody Abstract)
Background MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
Synonyms: Nuclear protein BM28, Minichromosome maintenance protein 2 homolog, DNA replication licensing factor MCM2, KIAA0030, CDCL1, CCNL1, BM28, MCM2
Molecular Weight 101896 DA
Gene ID 4171
UniProt P49736
Research Area Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Cell Structure
Pathways DNA Damage Repair
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.45 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Minichromosome Maintenance Complex Component 2 (MCM2) (AA 649-677), (C-Term) antibody (ABIN653324) Western blot analysis of MCM2 Antibody (C-term) (ABIN653324) in HL-60 cell line lysat...
Immunohistochemistry (IHC) image for anti-Minichromosome Maintenance Complex Component 2 (MCM2) (AA 649-677), (C-Term) antibody (ABIN653324) MCM2 Antibody (C-term) (ABIN653324) immunohistochemistry analysis in formalin fixed a...
Background publications Im, Ki, Farina et al.: "Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 37, pp. 15628-32, 2009 (PubMed).

Chuang, Teixeira, Wohlschlegel et al.: "Phosphorylation of Mcm2 by Cdc7 promotes pre-replication complex assembly during cell-cycle re-entry." in: Molecular cell, Vol. 35, Issue 2, pp. 206-16, 2009 (PubMed).

Liaw, Boyd: "MCM 2 Expression in Basal Cell Carcinomas and Trichoepitheliomas." in: Journal of cutaneous pathology, Vol. 36, Issue 10, pp. 1121-2, 2009 (PubMed).

Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).

Sugiyama, Masuda, Shinoda et al.: "Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 6, pp. 1103-9, 2007 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Catalog No. ABIN653324
$324.50
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