TGF-beta Activated Kinase 1/MAP3K7 Binding Protein 1 (TAB1) (Center) antibody

Antigen: TGF-beta Activated Kinase 1/MAP3K7 Binding Protein 1 (TAB1)

Synonyms: TAB1, 3'-Tab1, MGC57664

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653330

Additional Information

Alternative name: TAB1

Gene ID: 10454

UniProt: Q15750

Immunogen: This TAB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227~256 amino acids from the Center region of human TAB1.

Isotype: Ig

Description: Other names: TGF-beta-activated kinase 1-binding protein 1, TAK1-binding protein 1, Mitogen-activated protein kinase kinase kinase 7-interacting protein 1, TAB1, MAP3K7IP1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TAB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227~256 amino acids from the Center region of human TAB1.

Molecular Weight: 54644 DA

Comments:

Background: TAB1 was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. This protein interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of this protein is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. This protein can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of TAB1 Antibody (Center) (ABIN653330) in 293 cell line lysates (35ug/lane). TAB1 (arrow) was detected using the purified Pab.

TAB1 Antibody (Center) (ABIN653330) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TAB1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

TAB1 Antibody (Center) (ABIN653330) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Neil, Schiemann: "Altered TAB1:I kappaB kinase interaction promotes transforming growth factor beta-mediated nuclear factor-kappaB activation during breast cancer progression." in: Cancer research, Vol. 68, Issue 5, pp. 1462-70, 2008 (PubMed).

Prickett, Ninomiya-Tsuji, Broglie et al.: "TAB4 stimulates TAK1-TAB1 phosphorylation and binds polyubiquitin to direct signaling to NF-kappaB." in: The Journal of biological chemistry, Vol. 283, Issue 28, pp. 19245-54, 2008 (PubMed).

Isono, Kim, Ando et al.: "Suppression of cell invasiveness by periostin via TAB1/TAK1." in: International journal of oncology, Vol. 35, Issue 2, pp. 425-32, 2009 (PubMed).