GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1) (Center) antibody

Antigen: GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1)

Synonyms: GF1, GF-1, NFE1, XLTT, ERYF1, Gf-1, Gata-1, Eklf, Eryf1, GATA1, GATA transcription factor 1, gata-1, Gata1, si:dkeyp-77f7.5

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653457

Additional Information

Alternative name: GATA1

Gene ID: 2623

UniProt: P15976

Immunogen: This GATA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 279~308 amino acids from the Central region of human GATA1.

Isotype: Ig

Description: Other names: NF-E1 DNA-binding protein, GATA-binding factor 1, GATA-1, GF-1, Eryf1, Erythroid transcription factor, GF1, ERYF1, GATA1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GATA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 279~308 amino acids from the Central region of human GATA1.

Molecular Weight: 42751 DA

Comments:

Background: GATA1 is a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of GATA1 Antibody (Center) (ABIN653457) in K562 cell line lysates (35ug/lane). GATA1 (arrow) was detected using the purified Pab.

Publications

Product: Göbel, Taschner, Jurkin et al.: "Reciprocal role of GATA-1 and vitamin D receptor in human myeloid dendritic cell differentiation." in: Blood, Vol. 114, Issue 18, pp. 3813-21, 2009 (PubMed).

Hoeller, Bihl, Tzankov et al.: "New GATA1 mutation in codon 2 leads to the earliest known premature stop codon in transient myeloproliferative disorder." in: Blood, Vol. 114, Issue 17, pp. 3717-8, 2009 (PubMed).

Fujiwara, OGeen, Keles et al.: "Discovering hematopoietic mechanisms through genome-wide analysis of GATA factor chromatin occupancy." in: Molecular cell, Vol. 36, Issue 4, pp. 667-81, 2009 (PubMed).

Laurent, Randrianarison-Huetz, Maréchal et al.: "High-mobility group protein HMGB2 regulates human erythroid differentiation through trans-activation of GFI1B transcription." in: Blood, Vol. 115, Issue 3, pp. 687-95, 2010 (PubMed).