Hydroxyacylglutathione Hydrolase (HAGH) (C-Term) antibody

Antigen: Hydroxyacylglutathione Hydrolase (HAGH)

Synonyms: GLO2, GLX2, GLXII, HAGH1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653461

Additional Information

Alternative name: HAGH

Gene ID: 3029

UniProt: Q16775

Immunogen: This HAGH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 279~308 amino acids from the C-terminal region of human HAGH.

Isotype: Ig

Description: Other names: Glyoxalase II, Glx II, Hydroxyacylglutathione hydrolase, mitochondrial, HAGH1, GLO2, HAGH

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HAGH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 279~308 amino acids from the C-terminal region of human HAGH.

Molecular Weight: 33806 DA

Comments:

Background: HAGH is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of HAGH Antibody (C-term) (ABIN653461) in MDA-MB435 cell line lysates (35ug/lane). HAGH (arrow) was detected using the purified Pab.

Publications

Product: Limphong, McKinney, Adams et al.: "Human glyoxalase II contains an Fe(II)Zn(II) center but is active as a mononuclear Zn(II) enzyme." in: Biochemistry, Vol. 48, Issue 23, pp. 5426-34, 2009 (PubMed).

Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).