Interferon, gamma-Inducible Protein 30 (IFI30) (C-Term) antibody

Antigen: Interferon, gamma-Inducible Protein 30 (IFI30)

Synonyms: GILT, IP30, IFI-30, MGC32056, MGC124690, wu:fb12b04, zgc:101752, IFI30

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653470

Additional Information

Alternative name: IFI30

Gene ID: 10437

UniProt: P13284

Immunogen: This IFI30 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196~226 amino acids from the C-terminal region of human IFI30.

Isotype: Ig

Description: Other names: Gamma-interferon-inducible protein IP-30, Gamma-interferon-inducible lysosomal thiol reductase, GILT, IFI30

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This IFI30 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196~226 amino acids from the C-terminal region of human IFI30.

Molecular Weight: 29149 DA

Comments:

Background: IFI30 cleaves disulfide bonds in proteins by reduction. May facilitate the complete unfolding of proteins destined for lysosomal degradation. It may be involved in MHC class II-restricted antigen processing.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of IFI30 Antibody (C-term) (ABIN653470) in HepG2 cell line lysates (35ug/lane). IFI30 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with IFI30 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Imami, Sugiyama, Kyono et al.: "Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." in: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, Vol. 24, Issue 1, pp. 161-6, 2008 (PubMed).