Ephrin B3 (EFNB3) (Center) antibody

Antigen: Ephrin B3 (EFNB3)

Synonyms: EFL6, EPLG8, LERK8, efnb3-A, EphB3, EFNB3, Epl8, EFL-6, ELF-3, Elk-L3, LERK-8, NLERK-2, id:ibd5049, zgc:111938, ELK-L3, RGD1565678

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653624

Additional Information

Alternative name: EFNB3

Gene ID: 1949

UniProt: Q15768

Immunogen: This EFNB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97~127 amino acids from the Central region of human EFNB3.

Isotype: Ig

Description: Other names: EPH-related receptor transmembrane ligand ELK-L3, EPH-related receptor tyrosine kinase ligand 8, LERK-8, Ephrin-B3, LERK8, EPLG8, EFNB3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EFNB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97~127 amino acids from the Central region of human EFNB3.

Molecular Weight: 35835 DA

Comments:

Background: EFNB3, a member of the ephrin gene family, is important in brain development as well as in its maintenance. Moreover, since levels of EFNB3 expression were particularly high in several forebrain subregions compared to other brain subregions, it may play a pivotal role in forebrain function. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH Receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of EFNB3 Antibody (Center) (ABIN653624) in 293 cell line lysates (35ug/lane). EFNB3 (arrow) was detected using the purified Pab.

Publications

Product: Xu, Rajashankar, Chan et al.: "Host cell recognition by the henipaviruses: crystal structures of the Nipah G attachment glycoprotein and its complex with ephrin-B3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 29, pp. 9953-8, 2008 (PubMed).