K1H2 (Center) antibody

Antigen: K1H2

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653715

Additional Information

Gene ID: 3882

UniProt: Q14532

Immunogen: This K1H2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105~135 amino acids from the Central region of human K1H2 .

Isotype: Ig

Description: Other names: Keratin-32, K32, Hair keratin, type I Ha2, Keratin, type I cuticular Ha2,KRTHA2, HKA2, HHA2, KRT32

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This K1H2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105~135 amino acids from the Central region of human K1H2 .

Molecular Weight: 50319 DA

Comments:

Background: The protein encoded by this gene is a member of the keratin gene family. As a type I hair keratin, it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of K1H2 Antibody (Center) (ABIN653715) in K562 cell line lysates (35ug/lane). K1H2 (arrow) was detected using the purified Pab.

Publications

Product: Langbein, Rogers, Winter et al.: "The catalog of human hair keratins. I. Expression of the nine type I members in the hair follicle." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 19874-84, 1999 (PubMed).

Adkins, Varnum, Auberry et al.: "Toward a human blood serum proteome: analysis by multidimensional separation coupled with mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 1, Issue 12, pp. 947-55, 2003 (PubMed).

Schweizer, Bowden, Coulombe et al.: "New consensus nomenclature for mammalian keratins." in: The Journal of cell biology, Vol. 174, Issue 2, pp. 169-74, 2006 (PubMed).