Zinc Finger Protein 471 (ZNF471) (Center) antibody

Antigen: Zinc Finger Protein 471 (ZNF471)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN653732

Additional Information

Alternative name: ZNF471

Gene ID: 57573

UniProt: Q9BX82

Immunogen: This ZNF471 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140~170 amino acids from the Central region of human ZNF471.

Isotype: Ig

Description: Other names: EZFIT-related protein 1, Zinc finger protein 471£¬KIAA1396, ERP1, ZNF471

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ZNF471 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140~170 amino acids from the Central region of human ZNF471.

Molecular Weight: 73009 DA

Comments:

Background: ZNF471 belongs to the krueppel C2H2-type zinc-finger protein family. It contains fifteen C2H2-type zinc fingers and one KRAB domain. ZNF471 may be involved in transcriptional regulation.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of ZNF471 Antibody (Center) (ABIN653732) in HepG2 cell line lysates (35ug/lane). ZNF471 (arrow) was detected using the purified Pab.

ZNF471 Antibody (Center) (ABIN653732) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZNF471 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

ZNF471 Antibody (Center) (ABIN653732) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Grimwood, Gordon, Olsen et al.: "The DNA sequence and biology of human chromosome 19." in: Nature, Vol. 428, Issue 6982, pp. 529-35, 2004 (PubMed).