F-Box and WD Repeat Domain Containing 2 (FBXW2) (Center) antibody

Antigen: F-Box and WD Repeat Domain Containing 2 (FBXW2)

Synonyms: Md6, FBW2, Fwd2, MGC117371, MD6, 2700071L08Rik, fbxw2, MGC53244, FBXW2, md6, fbw2, fwd2

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN653738

Additional Information

Alternative name: FBXW2

Gene ID: 26190

UniProt: Q9UKT8

Immunogen: This FBXW2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119~149 amino acids from the Central region of human FBXW2.

Isotype: Ig

Description: Other names: Protein MD6, F-box and WD-40 domain-containing protein 2, F-box/WD repeat-containing protein 2, FWD2, FBW2, FBXW2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This FBXW2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119~149 amino acids from the Central region of human FBXW2.

Molecular Weight: 51512 DA

Comments:

Background: F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. Mammalian F-box proteins are classified into three groups based on the presence of either WD-40 repeats, leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. This gene encodes the second identified member of the F-box gene family and contains multiple WD-40 repeats.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of FBXW2 Antibody (Center) (ABIN653738) in HepG2 cell line lysates (35ug/lane). FBXW2 (arrow) was detected using the purified Pab.

FBXW2 Antibody (Center) (ABIN653738) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXW2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

FBXW2 Antibody (Center) (ABIN653738) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Busino, Donzelli, Chiesa et al.: "Degradation of Cdc25A by beta-TrCP during S phase and in response to DNA damage." in: Nature, Vol. 426, Issue 6962, pp. 87-91, 2003 (PubMed).

Watanabe, Arai, Nishihara et al.: "M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCFbeta-TrCP." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 13, pp. 4419-24, 2004 (PubMed).