Caspase Recruitment Domain Family, Member 8 (CARD8) (N-Term) antibody

Antigen: Caspase Recruitment Domain Family, Member 8 (CARD8)

Synonyms: NDPP, DACAR, Dakar, NDPP1, TUCAN, CARDINAL, FLJ18119, FLJ18121, KIAA0955, MGC57162, DKFZp779L0366, CARD8, DKFZp468E032

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN653820

Additional Information

Alternative name: CARD8

Gene ID: 22900

UniProt: Q9Y2G2

Immunogen: This CARD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39~68 amino acids from the N-terminal region of human CARD8.

Isotype: Ig

Description: Other names: Tumor up-regulated CARD-containing antagonist of CASP9, TUCAN, CARD-inhibitor of NF-kappa-B-activating ligand, CARDINAL, DACAR, Apoptotic protein NDPP1, Caspase recruitment domain-containing protein 8,NDPP1, KIAA0955, CARD8

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CARD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39~68 amino acids from the N-terminal region of human CARD8.

Molecular Weight: 48933 DA

Comments:

Background: CARD8 encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CARD8 Antibody (N-term) (ABIN653820) in HepG2 cell line lysates (35ug/lane). CARD8 (arrow) was detected using the purified Pab.

CARD8 Antibody (N-term) (ABIN653820) IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CARD8 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

CARD8 Antibody (N-term) (ABIN653820) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Agostini, Martinon, Burns et al.: "NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder." in: Immunity, Vol. 20, Issue 3, pp. 319-25, 2004 (PubMed).

Checinska, Giaccone, Hoogeland et al.: "TUCAN/CARDINAL/CARD8 and apoptosis resistance in non-small cell lung cancer cells." in: BMC cancer, Vol. 6, pp. 166, 2006 (PubMed).

Möckelmann, von Schönfels, Buch et al.: "Investigation of innate immunity genes CARD4, CARD8 and CARD15 as germline susceptibility factors for colorectal cancer." in: BMC gastroenterology, Vol. 9, pp. 79, 2009 (PubMed).

Hassan, Feyen, Grinstein: "Fas-induced apoptosis of renal cell carcinoma is mediated by apoptosis signal-regulating kinase 1 via mitochondrial damage-dependent caspase-8 activation." in: Cellular oncology : the official journal of the International Society for Cellular Oncology, Vol. 31, Issue 6, pp. 437-56, 2009 (PubMed).