F-Box and Leucine-Rich Repeat Protein 7 (FBXL7) (N-Term) antibody

Antigen: F-Box and Leucine-Rich Repeat Protein 7 (FBXL7)

Synonyms: FBL6, FBL7, Fbl6, AL023057, MGC102204, D230018M15Rik, FBXL7

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653834

Additional Information

Alternative name: FBXL7

Gene ID: 23194

UniProt: Q9UJT9

Immunogen: This FBXL7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98~127 amino acids from the N-terminal region of human FBXL7.

Isotype: Ig

Description: Other names: F-box protein FBL6/FBL7, F-box and leucine-rich repeat protein 7, F-box/LRR-repeat protein 7,KIAA0840, FBL7, FBL6, FBXL7

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This FBXL7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98~127 amino acids from the N-terminal region of human FBXL7.

Molecular Weight: 54575 DA

Comments:

Background: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of FBXL7 Antibody (N-term) (ABIN653834) in mouse heart tissue lysates (35ug/lane). FBXL7 (arrow) was detected using the purified Pab.

FBXL7 Antibody (N-term) (ABIN653834) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of FBXL7 (arrow) using rabbit polyclonal FBXL7 Antibody (N-term) (ABIN653834). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FBXL7 gene.

Publications

Product: Watanabe, Arai, Nishihara et al.: "M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCFbeta-TrCP." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 13, pp. 4419-24, 2004 (PubMed).

Venables, Bourgeois, Dalgliesh et al.: "Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon." in: Human molecular genetics, Vol. 14, Issue 16, pp. 2289-303, 2005 (PubMed).