Fibroblast Growth Factor 3 (FGF3) (C-Term) antibody

Antigen: Fibroblast Growth Factor 3 (FGF3)

Synonyms: INT2, HBGF-3, Fgf-3, Int-2, Int-P

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN653835

Additional Information

Alternative name: FGF3

Gene ID: 2248

UniProt: P11487

Immunogen: This FGF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203~232 amino acids from the C-terminal region of human FGF3.

Isotype: Ig

Description: Other names: Heparin-binding growth factor 3, HBGF-3, Proto-oncogene Int-2, Fibroblast growth factor 3, FGF-3,INT2, FGF3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This FGF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203~232 amino acids from the C-terminal region of human FGF3.

Molecular Weight: 26887 DA

Comments:

Background: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its similarity with mouse fgf3/int-2, a proto-oncogene activated in virally induced mammary tumors in the mouse. Frequent amplification of this gene has been found in human tumors, which may be important for neoplastic transformation and tumor progression. Studies of the similar genes in mouse and chicken suggested the role in inner ear formation.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of FGF3 Antibody (C-term) (ABIN653835) in MCF-7 cell line lysates (35ug/lane). FGF3 (arrow) was detected using the purified Pab.

FGF3 Antibody (C-term) (ABIN653835) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FGF3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

FGF3 Antibody (C-term) (ABIN653835) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Alsmadi, Meyer, Alkuraya et al.: "Syndromic congenital sensorineural deafness, microtia and microdontia resulting from a novel homoallelic mutation in fibroblast growth factor 3 (FGF3)." in: European journal of human genetics : EJHG, Vol. 17, Issue 1, pp. 14-21, 2008 (PubMed).

Charles, Lazzari, Tafforeau et al.: "Modulation of Fgf3 dosage in mouse and men mirrors evolution of mammalian dentition." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 52, pp. 22364-8, 2010 (PubMed).