DEN1A (C-Term), (AA 576-605) antibody

Details for Product No. ABIN653873
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Antigen
Epitope
C-Term, AA 576-605
Reactivity
Human
Host
Rabbit
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
Pubmed 2 references available
Quantity 400 μL
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Catalog No. ABIN653873
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Immunogen This DEN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 576-605 AA from the C-terminal region of human DEN1A.
Clone RB25211
Isotype Ig
Specificity This DEN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 583~613 amino acids from the C-terminal region of human DEN1A.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Background Synonyms: Protein FAM31A, Connecdenn, DENN domain-containing protein 1A,KIAA1608, FAM31A, DENND1A
Molecular Weight 110577 DA
Gene ID 57706
UniProt Q8TEH3
Research Area Phospho-specific antibodies, Cell Structure, Protein Modifications, Neurology
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-DEN1A (C-Term), (AA 576-605) antibody Western blot analysis of DEN1A Antibody (C-term) (ABIN653873) in K562 cell line lysates (35 µg/lane). DEN1A (arrow) was detected using the purified polyclonal antibody.
anti-DEN1A (C-Term), (AA 576-605) antibody (2) DEN1A Antibody (C-term) (ABIN653873) IHC analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining
anti-DEN1A (C-Term), (AA 576-605) antibody (3) DEN1A Antibody (C-term) (ABIN653873) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Allaire, Ritter, Thomas et al.: "Connecdenn, a novel DENN domain-containing protein of neuronal clathrin-coated vesicles functioning in synaptic vesicle endocytosis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 51, pp. 13202-12, 2006 (PubMed).

Validation Images
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