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Histone Cluster 3, H3 (HIST3H3) (Lys9) antibody

Details for Product No. ABIN653888, Supplier: Login to see
Antigen
  • H3.4
  • H3/g
  • H3FT
  • H3t
  • CG33848
  • Dmel\CG33848
  • H3.2-221
  • Hist1
  • H3/i
  • H3FI
  • h3
  • Hist1h3f
  • hist2h3c
  • T6J4.12
  • T6J4_12
Epitope
Lys9
113
107
75
55
50
39
36
34
32
31
29
28
27
25
25
24
23
22
22
20
20
19
17
17
16
16
16
16
16
16
15
14
14
13
13
13
13
12
11
11
10
10
10
9
9
9
9
8
7
7
7
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1203
553
494
166
41
36
35
28
26
17
14
12
9
7
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
1057
193
17
12
1
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
35
28
27
24
21
21
18
15
15
10
10
10
10
10
10
4
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1023
355
297
296
234
229
178
125
109
89
60
55
37
36
16
6
3
2
1
1
1
Supplier
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Immunogen This Histone H3-K9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the K9 region of human histone H3.
Clone RB08332
Isotype Ig
Specificity This Histone H3-K9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the K9 region of human histone H3.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name Histone H3 (HIST3H3 Antibody Abstract)
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene for histone H3-MeK9 is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Synonyms: Histone H3, PP781, H3F3, H3.3A, H3F3A
Molecular Weight 15273 DA
Gene ID 3021
UniProt P84243
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Pathways
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.32 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Histone Cluster 3, H3 (HIST3H3) (Lys9) antibody (ABIN653888) Western blot analysis of Methyl-K-H3-K9(2Me)-4MAPS (ABIN653888) in K562 cell line lys...
Immunohistochemistry (IHC) image for anti-Histone Cluster 3, H3 (HIST3H3) (Lys9) antibody (ABIN653888) Histone H3-K9 Antibody (ABIN653888) IHC analysis in formalin fixed and paraffin embed...
Flow Cytometry (FACS) image for anti-Histone Cluster 3, H3 (HIST3H3) (Lys9) antibody (ABIN653888) Histone H3-K9 Antibody (ABIN653888) flow cytometric analysis of K562 cells (bottom hi...
Background publications Teng, Ballim, Mowla et al.: "Phosphorylation of histone H3 by protein kinase C signaling plays a critical role in the regulation of the developmentally important TBX2 gene." in: The Journal of biological chemistry, Vol. 284, Issue 39, pp. 26368-76, 2009 (PubMed).

Garcia, Hake, Diaz et al.: "Organismal differences in post-translational modifications in histones H3 and H4." in: The Journal of biological chemistry, Vol. 282, Issue 10, pp. 7641-55, 2007 (PubMed).

Morris, Rao, Garcia et al.: "Identification of histone H3 lysine 36 acetylation as a highly conserved histone modification." in: The Journal of biological chemistry, Vol. 282, Issue 10, pp. 7632-40, 2007 (PubMed).

Loyola, Bonaldi, Roche et al.: "PTMs on H3 variants before chromatin assembly potentiate their final epigenetic state." in: Molecular cell, Vol. 24, Issue 2, pp. 309-16, 2006 (PubMed).