Histone Cluster 3, H3 (HIST3H3) (Lys9) antibody

Details for Product No. ABIN653888
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms H3.4, H3/g, H3FT, H3t, CG33848, Dmel\CG33848, H3.2-221, Hist1, H3/i, H3FI, h3, Hist1h3f, hist2h3c, T6J4.12, T6J4_12
Epitope
Lys9
(56), (49), (39), (31), (30), (24), (24), (24), (21), (20), (19), (18), (18), (17), (17), (16), (15), (11), (10), (10), (10), (10), (9), (8), (8), (8), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(689), (409), (404), (180), (169), (12), (7), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(626), (81), (6), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(29), (21), (20), (18), (17), (17), (17), (17), (17), (17), (17), (11), (11), (11), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(515), (224), (183), (181), (169), (94), (63), (59), (48), (38), (28), (8), (1), (1)
Pubmed 4 references available
Quantity 400 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN653888
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This Histone H3-K9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the K9 region of human histone H3.
Clone RB08332
Isotype Ig
Specificity This Histone H3-K9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the K9 region of human histone H3.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name Histone H3
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene for histone H3-MeK9 is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Synonyms: Histone H3, PP781, H3F3, H3.3A, H3F3A
Molecular Weight 15273 DA
Gene ID 3021
UniProt P84243
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.32 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Histone Cluster 3, H3 (HIST3H3) (Lys9) antibody Western blot analysis of Methyl-K-H3-K9(2Me)-4MAPS (ABIN653888) in K562 cell line lysates (35 µg/lane). H3 (arrow) was detected using the purified polyclonal antibody.
anti-Histone Cluster 3, H3 (HIST3H3) (Lys9) antibody (2) Histone H3-K9 Antibody (ABIN653888) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining
anti-Histone Cluster 3, H3 (HIST3H3) (Lys9) antibody (3) Histone H3-K9 Antibody (ABIN653888) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Loyola, Bonaldi, Roche et al.: "PTMs on H3 variants before chromatin assembly potentiate their final epigenetic state." in: Molecular cell, Vol. 24, Issue 2, pp. 309-16, 2006 (PubMed).

Morris, Rao, Garcia et al.: "Identification of histone H3 lysine 36 acetylation as a highly conserved histone modification." in: The Journal of biological chemistry, Vol. 282, Issue 10, pp. 7632-40, 2007 (PubMed).

Garcia, Hake, Diaz et al.: "Organismal differences in post-translational modifications in histones H3 and H4." in: The Journal of biological chemistry, Vol. 282, Issue 10, pp. 7641-55, 2007 (PubMed).

Teng, Ballim, Mowla et al.: "Phosphorylation of histone H3 by protein kinase C signaling plays a critical role in the regulation of the developmentally important TBX2 gene." in: The Journal of biological chemistry, Vol. 284, Issue 39, pp. 26368-76, 2009 (PubMed).

Validation Images
Did you look for something else?
back to top