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Histone H3 (Lys9) antibody

Antigen

Histone H3
Synonyms
H3t, H3.4, H3/g, H3FT, MGC126886, MGC126888, Hist1, H3.2-221, DmelCG31613, CG31613, h3.2, h3/m, h3f2, h3fm, hist1h3g, H3F3, H3.3A, H3F3B, MGC87782, MGC87783, H3.3B, H3F3A, h3f3a, MGC52829, MGC86731, M ... show more
Binding Site
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
4 references available
Certificates ISO 9001:2008
Catalog no. ABIN653888
Quantity 0.1 mg  (0.25 mg/ml)
Price 280.50 $   Plus shipping costs $45.00
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anti-Histone H3 (Lys9) antibody

anti-Histone H3 (Lys9) antibody (2)

anti-Histone H3 (Lys9) antibody (3)

Additional Information
Application Details Publications Images Alternatives back to top

Gene ID 3021
UniProt P84243
Immunogen This Histone H3-K9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the K9 region of human histone H3.
Isotype Ig
Description Other names: Histone H3, PP781, H3F3, H3.3A, H3F3A
Characteristics Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Specificity This Histone H3-K9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the K9 region of human histone H3.
Molecular Weight 15273 DA
Comments

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene for histone H3-MeK9 is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Synonyms H3t, H3.4, H3/g, H3FT, MGC126886, MGC126888, Hist1, H3.2-221, DmelCG31613, CG31613, h3.2, h3/m, h3f2, h3fm, hist1h3g, H3F3, H3.3A, H3F3B, MGC87782, MGC87783, H3.3B, H3F3A, h3f3a, MGC52829, MGC86731, MGC165454, zgc:56193, zgc:86731, wu:fb58e10, FLJ35099, FLJ56780, FLJ56787, MGC131639, MGC89877, HIST1H3A

Application Details
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Application Notes The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50
Concentration 0.25 mg/ml
Purification Purified
Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Restrictions For Research Use only

Publications
Application Details Images Alternatives back to top

Product Loyola, Bonaldi, Roche et al.: "PTMs on H3 variants before chromatin assembly potentiate their final epigenetic state." in: Molecular cell, Vol. 24, Issue 2, pp. 309-16, 2006 (PubMed).

Morris, Rao, Garcia et al.: "Identification of histone H3 lysine 36 acetylation as a highly conserved histone modification." in: The Journal of biological chemistry, Vol. 282, Issue 10, pp. 7632-40, 2007 (PubMed).

Garcia, Hake, Diaz et al.: "Organismal differences in post-translational modifications in histones H3 and H4." in: The Journal of biological chemistry, Vol. 282, Issue 10, pp. 7641-55, 2007 (PubMed).

Teng, Ballim, Mowla et al.: "Phosphorylation of histone H3 by protein kinase C signaling plays a critical role in the regulation of the developmentally important TBX2 gene." in: The Journal of biological chemistry, Vol. 284, Issue 39, pp. 26368-76, 2009 (PubMed).

Alternatives
Application Details Publications Images back to top

Alternatives for antigen "Histone H3", type "Antibodies"
Hosts Rabbit (188), Mouse (26), Sheep (6)
Reactivities Human (196), Rat (Rattus) (91), Mouse (Murine) (82), Cow (Bovine) (42), Fruit Fly (Drosophila melanogaster) (38), Pig (Porcine) (38), All Species (1), Arabidopsis thaliana (1), Mammalian (1), Rabbit (1), Rice (1), Tetrahymena (1)
Applications Western Blotting (WB) (149), Immunofluorescence (IF) (88), ELISA (86), Immunohistochemistry (IHC) (45), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (27), Immunocytochemistry (ICC) (23), Immunoprecipitation (IP) (19), Flow Cytometry (FACS) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Functional Studies (Func) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 488 (8), Alexa Fluor 350 (7), Alexa Fluor 555 (7), Alexa Fluor 647 (7), PE,Cy5.5 (7), Agarose Beads (2), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer10 (25), N-Term (17), pSer28 (17), pThr11 (13), pThr3 (10), C-Term (7), 2meLys9 (6), 3meLys4 (4), Lys27 (4), acLys14 (4), acLys9 (4), meLys9 (4), Lys4 (3), meLys4 (3), 2meLys27 (2), 2meLys4 (2), 3meLys27 (2), Lys36 (2), Mono Methyl Lysine 4 (2), Mono/bis-Citrulline 2-8-17 (2), Tri Methyl Lysine 4 (2), acLys18 (2), acLys23 (2), acLys27 (2), acLys56 (2), acetylated (2), ciArg2, ciArg8, ciArg17 (2), 2meArg2 (1), 2meArg8 (1), 2meLys4, 2meLys9 (1), 3meLys36 (1), 3meLys9 (1), AA 66-82 (1), AA 7-20 (1), Arg2 (1), Lys14,Lys9 (1), Lys79 (1), Lys9 (1), N Term, asymmetric dimethyl Arg2 (R2ADMA) (1), Ser10 (1), meLys9, 2meLys9, 3meLys9 (1), pSer10,pSer28 (1), pSer140 (1), pSer24,pSer20 (1)