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Histone H3 (Lys9) antibody
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
|4 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This Histone H3-K9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the K9 region of human histone H3.|
|Description||Other names: Histone H3, PP781, H3F3, H3.3A, H3F3A|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This Histone H3-K9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the K9 region of human histone H3.|
|Molecular Weight||15273 DA|
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene for histone H3-MeK9 is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
|Synonyms||H3t, H3.4, H3/g, H3FT, MGC126886, MGC126888, Hist1, H3.2-221, DmelCG31613, CG31613, h3.2, h3/m, h3f2, h3fm, hist1h3g, H3F3, H3.3A, H3F3B, MGC87782, MGC87783, H3.3B, H3F3A, h3f3a, MGC52829, MGC86731, MGC165454, zgc:56193, zgc:86731, wu:fb58e10, FLJ35099, FLJ56780, FLJ56787, MGC131639, MGC89877, HIST1H3A|
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Loyola, Bonaldi, Roche et al.: "PTMs on H3 variants before chromatin assembly potentiate their final epigenetic state." in: Molecular cell, Vol. 24, Issue 2, pp. 309-16, 2006 (PubMed).
Morris, Rao, Garcia et al.: "Identification of histone H3 lysine 36 acetylation as a highly conserved histone modification." in: The Journal of biological chemistry, Vol. 282, Issue 10, pp. 7632-40, 2007 (PubMed).
Garcia, Hake, Diaz et al.: "Organismal differences in post-translational modifications in histones H3 and H4." in: The Journal of biological chemistry, Vol. 282, Issue 10, pp. 7641-55, 2007 (PubMed).
Teng, Ballim, Mowla et al.: "Phosphorylation of histone H3 by protein kinase C signaling plays a critical role in the regulation of the developmentally important TBX2 gene." in: The Journal of biological chemistry, Vol. 284, Issue 39, pp. 26368-76, 2009 (PubMed).