Uracil-DNA Glycosylase (UNG) (C-Term) antibody

Antigen: Uracil-DNA Glycosylase (UNG)

Synonyms: MGC108922, DGU, UDG, UNG1, UNG2, HIGM4, UNG15, DKFZp781L1143, UNG, MGC140717, MGC56102, zgc:56102, ECK2578, JW2564, SPS, MGC82673

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN653892

Additional Information

Alternative name: UNG

Gene ID: 7374

UniProt: P13051

Immunogen: This UNG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249~278 amino acids from the C-terminal region of human UNG.

Isotype: Ig

Description: Other names: Uracil-DNA glycosylase, UDG,UNG15, UNG1, DGU, UNG

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This UNG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249~278 amino acids from the C-terminal region of human UNG.

Molecular Weight: 34645 DA

Comments:

Background: UNG encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Phospho-specific antibodies, Cell Signaling, Stem Cells, Protein Modifications, Chromatin, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of UNG Antibody (C-term) (ABIN653892) in T47D cell line lysates (35ug/lane). UNG (arrow) was detected using the purified Pab.

UNG Antibody (C-term)(ABIN653892) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the UNG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

UNG Antibody (C-term) (ABIN653769) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Ameratunga, Woon: "Customised molecular diagnosis of primary immune deficiency disorders in New Zealand: an efficient strategy for a small developed country." in: The New Zealand medical journal, Vol. 122, Issue 1304, pp. 46-53, 2009 (PubMed).