Kinesin Light Chain 4 (KLC4) (N-Term) antibody

Antigen: Kinesin Light Chain 4 (KLC4)

Synonyms: DKFZp459H1717

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653901

Additional Information

Alternative name: KLC4

Gene ID: 89953

UniProt: Q9NSK0

Immunogen: This KLC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92~121 amino acids from the N-terminal region of human KLC4.

Isotype: Ig

Description: Other names: Kinesin-like protein 8, Kinesin light chain 4, KLC 4,KNSL8, KLC4

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This KLC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92~121 amino acids from the N-terminal region of human KLC4.

Molecular Weight: 68640 DA

Comments:

Background: KLC4 is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity. The oligomeric complex is composed of two heavy chains and two light chains.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of KLC4 Antibody (N-term) (ABIN653901) in Hela cell line lysates (35ug/lane). KLC4 (arrow) was detected using the purified Pab.

KLC4 Antibody (N-term) (ABIN653901) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Sugiyama, Masuda, Shinoda et al.: "Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 6, pp. 1103-9, 2007 (PubMed).