NAL11 (Center) antibody

Antigen: NAL11

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN653949

Additional Information

Gene ID: 204801

UniProt: P59045

Immunogen: This NAL11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 467~497 amino acids from the Central region of human NAL11.

Isotype: Ig

Description: Other names: PAAD-and NACHT domain-containing protein 10, Nucleotide-binding oligomerization domain protein 17, PYRIN-containing APAF1-like protein 6, NACHT, LRR and PYD domains-containing protein 11,PYPAF6, PAN10, NOD17, NALP11, NLRP11

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NAL11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 467~497 amino acids from the Central region of human NAL11.

Molecular Weight: 117779 DA

Comments:

Background: NAL11 are cytoplasmic proteins that form a subfamily within the larger CATERPILLER protein family. Most short NALPs, such as NALP11, have an N-terminal pyrin (MEFV, MIM 608107) domain (PYD), followed by a NACHT domain, a NACHT-associated domain (NAD), and a C-terminal leucine-rich repeat (LRR) region. The long NALP, NALP1 (MIM 606636), also has a C-terminal extension containing a function to find domain (FIIND) and a caspase recruitment domain (CARD). NAL11 are implicated in the activation of proinflammatory caspases (e.g., CASP1, MIM 147678) via their involvement in multiprotein complexes called inflammasomes.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of NAL11 Antibody (Center) (ABIN653949) in HL-60 cell line lysates (35ug/lane). NAL11 (arrow) was detected using the purified Pab.

NAL11 Antibody (Center) (ABIN653949) IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NAL11 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

NAL11 Antibody (Center) (ABIN653949) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Dowds, Masumoto, Chen et al.: "Regulation of cryopyrin/Pypaf1 signaling by pyrin, the familial Mediterranean fever gene product." in: Biochemical and biophysical research communications, Vol. 302, Issue 3, pp. 575-80, 2003 (PubMed).

Baranzini, Wang, Gibson et al.: "Genome-wide association analysis of susceptibility and clinical phenotype in multiple sclerosis." in: Human molecular genetics, Vol. 18, Issue 4, pp. 767-78, 2009 (PubMed).

Enjuanes, Benavente, Bosch et al.: "Genetic variants in apoptosis and immunoregulation-related genes are associated with risk of chronic lymphocytic leukemia." in: Cancer research, Vol. 68, Issue 24, pp. 10178-86, 2008 (PubMed).