Adenosylmethionine Decarboxylase 1 (AMD1) (C-Term) antibody

Antigen: Adenosylmethionine Decarboxylase 1 (AMD1)

Synonyms: AMD, SAMDC, ADOMETDC, FLJ26964, DKFZp313L1234, Amd1a, Amd1b, AMD1, amd, samdc, AdoMetDC, MGC53431, MGC55614, MGC191134, zgc:55614, si:ch211-257g8.2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654059

Additional Information

Alternative name: AMD1

Gene ID: 262

UniProt: P17707, NP_001625.2

Immunogen: This AMD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-317 amino acids from the C-terminal region of human AMD1.

Isotype: Ig

Description: Other names: S-adenosylmethionine decarboxylase proenzyme,AMD1,AMD

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This AMD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-317 amino acids from the C-terminal region of human AMD1.

Molecular Weight: 38340 DA

Comments:

Background: This gene encodes an important intermediate enzyme in polyamine biosynthesis. The polyamines spermine, spermidine, and putrescine are low-molecular-weight aliphatic amines essential for cellular proliferation and tumor promotion. Two alternatively spliced transcript variants that encode different proteins have been identified. Pseudogenes of this gene are found on chromosomes 5, 6, 10, X and Y.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

AMD1 Antibody (C-term) (ABIN654059) western blot analysis in MCF-7 cell line lysates (35ug/lane). This demonstrates the AMD1 antibody detected the AMD1 protein (arrow).

Publications

Product: Bale, Lopez, Makhatadze et al.: "Structural basis for putrescine activation of human S-adenosylmethionine decarboxylase." in: Biochemistry, Vol. 47, Issue 50, pp. 13404-17, 2008 (PubMed).

Franke, Vermeulen, Steegers-Theunissen et al.: "An association study of 45 folate-related genes in spina bifida: Involvement of cubilin (CUBN) and tRNA aspartic acid methyltransferase 1 (TRDMT1)." in: Birth defects research. Part A, Clinical and molecular teratology, Vol. 85, Issue 3, pp. 216-26, 2009 (PubMed).

Levine, Figueiredo, Lee et al.: "A candidate gene study of folate-associated one carbon metabolism genes and colorectal cancer risk." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 19, Issue 7, pp. 1812-21, 2010 (PubMed).