SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4) (N-Term) antibody

Antigen: SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4)

Synonyms: BRG1, SNF2, SWI2, BAF190, SNF2L4, SNF2LB, hSNF2b, FLJ39786, SNF2-BETA, SMARCA4

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654081

Additional Information

Alternative name: BRG1

Gene ID: 6597

UniProt: NP_003063.2, NP_001122316.1, P51532

Immunogen: This BRG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-98 amino acids from the N-terminal region of human BRG1.

Isotype: Ig

Description: Other names: Transcription activator BRG1,SMARCA4,BAF190A, BRG1, SNF2B, SNF2L4

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BRG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-98 amino acids from the N-terminal region of human BRG1.

Molecular Weight: 184646 DA

Comments:

Background: The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Neurology, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

BRG1 Antibody (N-term) (ABIN654081) western blot analysis in mouse cerebellum tissue lysates (15ug/lane). This demonstrates the BRG1 antibody detected BRG1 protein (arrow).

Publications

Product: Bakshi, Hassan, Pratap et al.: "The human SWI/SNF complex associates with RUNX1 to control transcription of hematopoietic target genes." in: Journal of cellular physiology, Vol. 225, Issue 2, pp. 569-76, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Alessio, Squillaro, Cipollaro et al.: "The BRG1 ATPase of chromatin remodeling complexes is involved in modulation of mesenchymal stem cell senescence through RB-P53 pathways." in: Oncogene, Vol. 29, Issue 40, pp. 5452-63, 2010 (PubMed).