DSN1, MIND Kinetochore Complex Component, Homolog (S. Cerevisiae) (DSN1) (Center) antibody

Antigen: DSN1, MIND Kinetochore Complex Component, Homolog (S. Cerevisiae) (DSN1)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Hamster

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654211

Additional Information

Alternative name: DSN1

Gene ID: 79980

UniProt: NP_001138788.1, Q9H410, NP_001138787.1, NP_079194.3

Immunogen: This DSN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-119 amino acids from the Central region of human DSN1.

Isotype: Ig

Description: Other names: Kinetochore-associated protein DSN1 homolog,DSN1,C20orf172, MIS13

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DSN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-119 amino acids from the Central region of human DSN1.

Molecular Weight: 40067 DA

Comments:

Background: This gene encodes a kinetochore protein that functions as part of the minichromosome instability-12 centromere complex. The encoded protein is required for proper kinetochore assembly and progression through the cell cycle. Alternative splicing results in multiple transcript variants.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

DSN1 Antibody (Center) (ABIN654211) western blot analysis in mouse CHO cell line lysates (35ug/lane). This demonstrates the DSN1 antibody detected the DSN1 protein (arrow).

Publications

Product: Lamesch, Li, Milstein et al.: "hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes." in: Genomics, Vol. 89, Issue 3, pp. 307-15, 2007 (PubMed).

Kiyomitsu, Obuse, Yanagida: "Human Blinkin/AF15q14 is required for chromosome alignment and the mitotic checkpoint through direct interaction with Bub1 and BubR1." in: Developmental cell, Vol. 13, Issue 5, pp. 663-76, 2007 (PubMed).

Cheeseman, Desai: "Molecular architecture of the kinetochore-microtubule interface." in: Nature reviews. Molecular cell biology, Vol. 9, Issue 1, pp. 33-46, 2007 (PubMed).

Yang, Wu, Ward et al.: "Phosphorylation of HsMis13 by Aurora B kinase is essential for assembly of functional kinetochore." in: The Journal of biological chemistry, Vol. 283, Issue 39, pp. 26726-36, 2008 (PubMed).