Lipase I (LIPI) (C-Term) antibody

Antigen: Lipase I (LIPI)

Synonyms: Liph, D930038D03Rik, lipi, lpdl, lpdlr, pla1b, pred5, mpa-pla1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654224

Additional Information

Alternative name: LIPI

Gene ID: 149998

UniProt: Q6XZB0, NP_945347.1

Immunogen: This LIPI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-388 amino acids from the C-terminal region of human LIPI.

Isotype: Ig

Description: Other names: Lipase member I,LIPI,LPDL

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This LIPI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-388 amino acids from the C-terminal region of human LIPI.

Molecular Weight: 52992 DA

Comments:

Background: The protein encoded by this gene is a phospholipase that hydrolyzes phosphatidic acid to produce lysophosphatidic acid. The encoded protein, which can be inhibited by sodium vanadate, may be found exclusively in sperm. Defects in this gene are a cause of susceptibility to familial hypertrigliceridemia. [provided by RefSeq].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

LIPI antibody(C-term) (ABIN654224) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LIPI antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Wen, Hegele, Wang et al.: "Identification of a novel lipase gene mutated in lpd mice with hypertriglyceridemia and associated with dyslipidemia in humans." in: Human molecular genetics, Vol. 12, Issue 10, pp. 1131-43, 2003 (PubMed).

Hiramatsu, Sonoda, Takanezawa et al.: "Biochemical and molecular characterization of two phosphatidic acid-selective phospholipase A1s, mPA-PLA1alpha and mPA-PLA1beta." in: The Journal of biological chemistry, Vol. 278, Issue 49, pp. 49438-47, 2003 (PubMed).

Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).