Oligonucleotide/oligosaccharide-Binding Fold Containing 2A (OBFC2A) (Center) antibody

Antigen: Oligonucleotide/oligosaccharide-Binding Fold Containing 2A (OBFC2A)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654244

Additional Information

Alternative name: OBFC2A

Gene ID: 64859

UniProt: Q96AH0, NP_001026886.1

Immunogen: This OBFC2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-134 amino acids from the Central region of human OBFC2A.

Isotype: Ig

Description: Other names: SOSS complex subunit B2,OBFC2A,SSB2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This OBFC2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-134 amino acids from the Central region of human OBFC2A.

Molecular Weight: 22423 DA

Comments:

Background: Single-stranded DNA (ssDNA)-binding proteins, such as OBFC2A, are ubiquitous and essential for a variety of DNA metabolic processes, including replication, recombination, and detection and repair of damage (Richard et al., 2008 [PubMed 18449195]).[supplied by OMIM].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

OBFC2A antibody (Center) (ABIN654244) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the OBFC2A antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Lamesch, Li, Milstein et al.: "hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes." in: Genomics, Vol. 89, Issue 3, pp. 307-15, 2007 (PubMed).

Richard, Bolderson, Cubeddu et al.: "Single-stranded DNA-binding protein hSSB1 is critical for genomic stability." in: Nature, Vol. 453, Issue 7195, pp. 677-81, 2008 (PubMed).

Li, Bolderson, Kumar et al.: "HSSB1 and hSSB2 form similar multiprotein complexes that participate in DNA damage response." in: The Journal of biological chemistry, Vol. 284, Issue 35, pp. 23525-31, 2009 (PubMed).

Huang, Gong, Ghosal et al.: "SOSS complexes participate in the maintenance of genomic stability." in: Molecular cell, Vol. 35, Issue 3, pp. 384-93, 2009 (PubMed).