NEDD4 Binding Protein 1 (N4BP1) (N-Term) antibody

Antigen: NEDD4 Binding Protein 1 (N4BP1)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654475

Additional Information

Alternative name: N4BP1

Gene ID: 9683

UniProt: NP_694574.3, O75113

Immunogen: This N4BP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-305 amino acids from the N-terminal region of human N4BP1.

Isotype: Ig

Description: Other names: N4BP1,NEDD4-binding protein 1,KIAA0615

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This N4BP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-305 amino acids from the N-terminal region of human N4BP1.

Molecular Weight: 100379 DA

Comments:

Background: Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates (By similarity).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

N4BP1 Antibody (N-term) (ABIN654475) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).

Enjuanes, Benavente, Bosch et al.: "Genetic variants in apoptosis and immunoregulation-related genes are associated with risk of chronic lymphocytic leukemia." in: Cancer research, Vol. 68, Issue 24, pp. 10178-86, 2008 (PubMed).